the Accuri C6 Flow Cytometer System. BD LSRFortessa 2. Amnis ImageStreamX. BD™ Accuri™ Bacteriostatic Concentrate Solution can be used to treat 10L of sheath fluid. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. The running costs are very low as it doesn¹t use any proprietary sheath fluid or reagents. Came with note that it needs repair. %%EOF … Such wear includes scratches, scrapes, and general dirtiness. 3. Hi, our Accuri C6 has stopped taking liquid. %PDF-1.6 %���� The BD Accuri C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. A compact optical design, fixed alignment, and pre- The ideal cell concentration 1230 York Avenue, Box 344 - Find MSDS or SDS, a COA, data sheets and more information. filters. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). To view hidden file extensions which distinguish BD Accuri C6 Software files (.c6) from BD Accuri C6 Software template files (.c6t): 1. BD LSRFortessa X-20. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. You are viewing an Accuri C6 Flow Cytometer. New York, NY 10065. Packaged in a pack of ten 8mL vials. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. Clinical Research and the Rockefeller University Hospital, Chemers Neustein Summer Undergraduate Research Fellowship Program, Experience Science, the Arts, and Culture. Contact, Flow Cytometry Resource Center (FCRC) BD Accuri™ C6 software was started. h�bbd``b`:$� -�8�`�$XJA�H�H0'��U �.+ The BD Accuri® C6 is a digital basic flow cytometer, which is equipped with a Blue (488nm) and a Red (640nm) lasers, two light scatter detectors (FSC and SSC), and four fluorescence detectors. BD offers a selction of inferior vena cava filter products. No. Click on “View” tab 4. Filter and Fluorochrome List for the Accuri C6 Flow Cytometer ® System As of: January 1, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP Filter and Fluorochrome List for the Accuri® C6 Flow Cytometer® System ©January, 2010 Standard Filters Optional Filters (other) Fluorescent Protein Filters (for simultaneous GFP/YFP detection) FL1 530 +/- 15nm FL1 510 +/- 7.5 nm 510 +/- 7.5 nm FL2 585 +/- 20 nm FL2 565 +/- 10 nm 540 +/- 15nm FL3 670nm LP FL3 610 +/- 10 nm 670nm LP Also the filters and lasers can be swapped out to suit your needs with easily available off-the-shelf substitutes (this was mentioned by one of the Accuri techs, I personally cannot verify this). For any system, we recommend: • Check all fluidic tanks for contamination and replace all cubitainers as applicable. Overall very good cosmetic condition. The Accuri C6 Flow Cytometer does not produce aerosols during normal operation. The latest science discoveries delivered monthly to your inbox. endstream endobj 30 0 obj <>>> endobj 31 0 obj <> endobj 32 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/Properties<>>>/Rotate 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 33 0 obj <>stream In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. ... considering that we still have to buy the replacement kit with filters, inline filter, and rubber tubbings. Easy to use - fixed alignment and pre-optimized detector settings. Researchers at the Swiss Federal Institute of Aquatic Science and Technology (Eawag, Switzerland) have developed a standard flow cytometric staining protocol and a corresponding BD AccuriTM C6 software analysis template to discriminate bacteria from debris in drinking water samples. Training can be requested via PPMS. ... BD Accuri C6. You can also set custom speeds/core sizes up to a 40 micron core, that might help the clogging issue with your 30 micron cells. All rights reserved. Copyright 2004—2020 The Rockefeller University. Light, rugged, and small enough to fit on a benchtop, it connects to standard electrical circuitry and can easily be transported to a water treatment facility or into the field. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Item is sold AS IS. ... filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. ... optical filters optimized for the detection of FITC, PE, PerCP, and APC. To increase signal resolution, or to separate fluorochromes with overlapping signals, a choice of optional, user-interchangeable filters is available. • Replace all fluid filters … Analytical Flow Cytometry - Accuri C6 Configuration Please note that the Accuri C6 is for use by trained personnel only! Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. 661085). With minor signs of normal wear and tear. Ē\�@BBH"v@��d� �Hz �H4�0012����H%�?�ݏ x�S Prevents the growth of bacteria in the sheath fluid of the Accuri™ C6 Flow Cytometer. C6 Flow Cytometer 1-year Maintenance Kit . filter, and bottle filters. Data is digitally collected over a 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. Since yeasts are relatively large (I use an Accuri C6 to count bacteria so yeast are large to me) I would go with the faster/larger core size. 653146, 653148, and 653147 Sample Preparation Volume measurements on the BD Accuri C6 are most accurate with cell concentrations between 1,000 and 5000000 cells/mL. Which fluorochromes are compatible with the cytometers? Table of Contents. Includes all accessories shown in pictures. BD Accuri® C6 is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. This item has been powered on, but no further testing. Nos. Designed from the ground up to be compact and easy to use, the BD Accuri C6 flow cytometer fits in any lab and is readily accessible Brand BD Accuri™ Contents Contains (12) Peristaltic Pump Tubing, (6) Sheath Bottle Filters, (12) Decon/Cleaner Bottle Filters and (6) In-Line Sheath Filters The BD Accuri C6 flow cytometer (Figure 2) offers performance, simplicity, and affordability. Always protect the Sample Introduction Probe (SIP) with a tube of HPLC reagent grade filtered, deionized H 2 O (0.22 µm filter), or equivalent, when the cytometer is not running samples. Standard fluorochrome in use are identical to FACS Calibur. Phase III+: The University is open for expanded research operations; only authorized personnel will be admitted on campus. FCRC Waiting List Forms Note: This is not a full list of all fluorochromes that are compatible with the flow cytometers. BD Accuri™ C6 Flow Cytometer Features: Equipped with a blue and a red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. BD Accuri® C6 CFlow SAMPLER supports the high throughput and fully automated acquisition from 48- and 96-well plates and deep-well plates. 52 0 obj <>/Filter/FlateDecode/ID[<6D17E613E92D6948AEF4F9A1572DFD51>]/Index[29 41]/Info 28 0 R/Length 106/Prev 60895/Root 30 0 R/Size 70/Type/XRef/W[1 2 1]>>stream 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. The Accuri C6 cytometer in my lab has some problems, and we need some help. The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. Cat. Rockefeller University Manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages before. And affordability by FCR staff are here: the University is open for expanded operations. 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